Protein Engineering Seed Grant

Applications accepted for Track 1 only (budget of $50,000)

Protein Engineering projects can include the selection of VL-based nanobodies by phage display. Optionally, the resulting clones (max. 5) will be fused to an Fc, produced and PA-purified. The clones will be analyzed for purity (PAGE, anal. SEC), binding (ELISA, SPR), cell binding and specificity (flow cytometry), and stability (DLS). Screening library options should be discussed with the Nucleus Director during the proposal development process. 

In-kind support will include access to the Protein Engineering laboratory to perform panning, screening, and optionally, cloning, production, purification, and characterization, as well as associated consumables. It will also include guidance and academic support from at least one member of the Protein Engineering laboratory.

Competitive projects will identify at least one graduate student or postdoctoral scholar who should expect to devote one month of full-time effort in the lab space at the Protein Engineering laboratory for panning and screening. An additional commitment of 25% time for 4 weeks is expected for optional nanobody production and characterization. Every project will be analyzed by a member of the Protein Engineering laboratory for feasibility and necessary tools to be provided by the PI’s laboratory.

Protein Engineering is led by Adrian Hugenmatter (adrian.hugenmatter@stanford.edu)