Equipment & Software
Equipment
The Metabolomics Knowledge Center (MKC) currently hosts three mass spectrometers: two high resolution accurate mass instruments for untargeted metabolomics and one triple quadrupole instrument for targeted analysis.
Agilent 6545 Q-TOF mass spectrometer with Agilent 1290 Infinity II UHPLC
A high resolution accurate mass Q-TOF instrument offers MS/MS capability and reliable isotopic fidelity. This instrument can be applied to broad applications such as untargeted metabolomics profiling, qualitative targeted flux analysis, small molecule accurate mass determination, structure characterization and elucidation.
Access Status: $33/hr for approved Stanford users (FY21 approved rate)
Agilent 6470 triple quadrupole with Agilent 1290 Infinity II UHPLC
A workhorse for targeted quantitation applications such as targeted metabolomics analysis and small molecule bioanalysis
Access Status: $31/hr for approved Stanford users (FY21 approved rate)
Thermo Orbitrap ID-X Tribrid mass spectrometer with Vanquish UHPLC
A high resolution accurate mass instrument that combines leading mass analyzer technology with Thermo Scientific AcquireX intelligent MSn data acquisition strategy. This instrument offers broad applications such as untargeted and targeted metabolomics analyses, small molecule accurate mass determination. The MSn capability offers great advantages to small molecule structure elucidation and identification.
Access Status: Collaborative R&D projects only
Software
Instrument and vendor specific software packages are available to support data analyses and facilitate data interpretation.
Qualitative analysis software
· Agilent MassHunter Qualitative Analysis
· Thermo Freestyle
Quantitative analysis software
· Agilent MassHunter Quantitative Analysis
· Thermo TraceFinder
Metabolomics feature finding, statistical and pathway analysis, and flux analysis software
· Agilent Profinder
· Agilent Mass Profiler Professional
· Agilent Lipid Annotator
· Agilent VistaFlux
· Thermo Compound Discoverer
· Thermo Lipid Search